May 05, 2025
NEET 2025 Biology Paper: Complete Difficulty Analysis
NEET 2025 exam done? What's next? Most of the students are now wondering how the Biology section of the paper was—after all, it comprises 50% of the total marks of the paper. This year's NEET 2025 Biology paper was moderate in difficulty, and to be honest, it did have some tricky questions. Though most questions were straight from NCERT, the challenging aspect was with some of the questions which were testing not merely memory but application. There were more questions from Class 12 biology than Class 11, with maximum questions from Inheritance, Plant Kingdom, Biotechnology, Ecosystem and Cell. If you find yourself uncertain of your performance, you're not alone—this year's paper tested clarity of concepts more than ever before.
Also check: NEET 2025 answer key download
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Comprehensive Analysis of NEET 2025 Biology Questions
The NEET 2025 exam has been successfully conducted offline (pen-and-paper) on 4th May,2025 at different exam centers in India and abroad. The Biology paper had 90 questions, with half of them for Botany and half for Zoology, comprising a total of 360 marks. All the questions were based on the Class 11 and Class 12 NCERT syllabus and were aimed at testing both knowledge of concepts and application skills. This NEET 2025 Biology paper analysis contains in-depth chapter-wise, class-wise, and difficulty-level analysis, which will be beneficial for students who are preparing for upcoming NEET exams.
The NEET 2025 Biology Paper strictly adhered to the NCERT syllabus, with the majority of questions originating from NCERT concepts and lines, as was the case with previous years. This analysis comprises in-depth chapter-wise, class-wise, and difficulty-level breakdown, which will assist students preparing for the upcoming NEET exams.
NEET 2025 Biology Paper Overview
The NEET 2025 Biology paper analysis tells us that the paper was of moderate difficulty level, with straightforward NCERT-based questions and some application-based, tricky ones. Going by the general NEET paper analysis, students considered questions from chapters such as Inheritance, Plant Physiology, and Pedigree Analysis difficult. The NEET paper review states that the questions were distributed equally between Class 11 and 12. This NEET exam analysis points to the fact that although the paper was moderate for some students, some tricky questions increased the NEET difficulty level, which will potentially reduce the overall cutoff.
Aspect
|
Details
|
Total number of questions
|
90(Botany+Zoology)
|
Question types
|
Multiple Choice Questions(MCQs)
|
Total marks
|
360
|
Format of questions
|
Assertion-Reason, Match the Column, Single line MCQ
|
How Tough Was the NEET 2025 Biology Paper?
The NEET 2025 Biology paper was moderately difficult, with a well-balanced mix of easy, moderate, and challenging questions from both Botany and Zoology. Keeping in mind the latest trends, the paper examined not only fact-based memorization of concepts but also the capacity to apply fundamental theories in actual concept-based questions. The paper consisted of a blend of direct NCERT-based questions and application-based questions,with a few tricky ones, and thus conceptual clarity was critical. Both Class 11 and 12 syllabi were divided proportionately, with a slight emphasis on Class 12 topics. Overall, the paper required a strategic approach, combining good conceptual knowledge with rapid analytical thinking.
Subject
|
Easy
|
Medium
|
Hard
|
Grand Total
|
Botany
|
20
|
16
|
14
|
50
|
Zoology
|
19
|
15
|
6
|
40
|
Overall Difficulty Level Analysis
|
39
|
31
|
20
|
90
|


How NEET 2025 Biology Questions Were Split Between Class 11 and 12
The NEET 2025 Biology paper had a balanced combination of questions from Class 11 and Class 12, providing equal coverage of both syllabi. In the Botany paper, approximately 60% of the questions were from Class 12 topics, showing a slight focus towards advanced concepts. On the other hand, Zoology had an equal portion of Class 11 and 12 topics. Apart from these subject-wise variations, the paper overall saw a nearly even distribution class-wise. This balanced strategy allowed students who prepared well throughout the year to perform their best.
Subject
|
Class 11th
|
Class 12th
|
11th %age
|
12th %age
|
Botany
|
23
|
27
|
51%
|
60%
|
Zoology
|
20
|
20
|
44%
|
44%
|
Class-wise Question Distribution
|
43
|
47
|
24%
|
26%
|


NEET 2025 Biology Paper Analysis According to Question Types
Subject
|
Single Choice MCQ
|
Statement I & Statement II
|
Multi Statement Based
|
Assertion and Reason
|
Match the column
|
Diagram Based
|
Grand Total
|
Botany
|
26
|
5
|
8
|
5
|
6
|
0
|
50
|
Zoology
|
19
|
2
|
10
|
1
|
5
|
3
|
40
|
Total
|
45
|
7
|
18
|
6
|
11
|
3
|
90
|
Chapter-Wise Distribution of NEET 2025 Biology Questions
The NEET 2025 Biology paper contained an equal-balanced range of questions on major chapters of both Class 11 and 12. Topics of higher weightage were Biotechnology, Molecular Basis of Inheritance and Ecosystem, Plant Kingdom, and Cell, which together formed a major portion of the paper. Chapters such as Biomolecules, Cell – The Unit of Life, and Biotechnology also had a higher number of questions.
The pattern closely followed NCERT, with emphasis on concept-based knowledge. Candidates who concentrated on learning the concepts managed to answer the paper properly. It is important for future candidates to focus on key topics during preparation after understanding chapter-wise trends.
A Closer Look at the NEET 2025 Biology Paper Analysis by the team of PrepMed
Surprisingly, some of the questions in the NEET 2025 Biology paper were identical to those discussed in PrepMed's live marathon sessions and AITS exams. This is evidence of the excellent standard kept by PrepMed's mock tests to make students proficient for difficult situations.
By Conducting mock tests in real exam conditions and making the students practice with confusing and conceptual questions, the PrepMed team made aspirants capable enough to face the real NEET 2025 exam paper. With the moderate level of difficulty in the Biology section, the PrepMed’s Expert team expects a decrease in overall cutoff slightly. A Biology score of about 340 can be expected to be safe this year.
NEET Biology 2025 Chapter wise Breakdown for Botany
Class
|
Chapter Name
|
Number of Questions
|
11th
|
Respiration in Plants
|
1
|
11th
|
The Living World
|
1
|
11th
|
Cell: The Unit of Life
|
4
|
11th
|
Morphology of Flowering Plants
|
4
|
11th
|
Plant Growth and Development
|
2
|
11th
|
Plant Kingdom
|
5
|
11th
|
Biological Classification
|
2
|
11th
|
Anatomy of Flowering Plants
|
1
|
11th
|
Photosynthesis in Higher Plants
|
2
|
11th
|
Cell Cycle and Cell Division
|
1
|
12th
|
Molecular Basis of Inheritance
|
7
|
12th
|
Organisms and Populations
|
2
|
12th
|
Biodiversity and Conservation
|
2
|
12th
|
Ecosystem
|
4
|
12th
|
Sexual Reproduction in Flowering Plants
|
5
|
12th
|
Microbes in Human Welfare
|
3
|
12th
|
Principles of Inheritance and Variation
|
4
|
Total
|
50
|
NEET Biology 2025 Chapter wise Breakdown for Zoology
Class
|
Chapter Name
|
Number of Questions
|
11th
|
Body Fluids and Circulation
|
2
|
11th
|
Excretory Products and their Elimination
|
2
|
11th
|
Structural Organisation in Animals (Animal Tissues)
|
3
|
11th
|
Animal Kingdom
|
4
|
11th
|
Biomolecules
|
4
|
11th
|
Chemical Coordination and Integration
|
5
|
12th
|
Human Reproduction
|
4
|
12th
|
Biotechnology and its Applications
|
4
|
12th
|
Human Health and Disease
|
4
|
12th
|
Evolution
|
1
|
12th
|
Biotechnology - Principles and Processes
|
6
|
12th
|
Reproductive Health
|
1
|
Total
|
40
|
NEET 2025 Botany: Topic-Wise detailed analysis

Conclusion: NEET 2025 Biology Paper – Detailed Difficulty Analysis
In summary, the NEET 2025 Biology paper had a moderate level of difficulty, with a balanced distribution of factual, conceptual, and application-based questions. The paper successfully examined a student's depth of knowledge over just fact-based memorization. Both Class 11 and Class 12 syllabi were well-balanced, and important chapters such as Biotechnology,Plant Kingdom and Sexual Reproduction in Flowering Plants had considerable weightage.
Though a few difficult questions tested time management and reasoning ability, the majority were NCERT-based. The paper overall required in-depth conceptual preparation and strategic revision. Identification of this difficulty pattern can help the future aspirants in improving their strategy and prioritizing high-weightage topics.
FAQS:
- Was the NEET 2025 Biology paper tougher than last year?
The NEET 2025 Biology paper was moderately difficult, similar to previous years, with a few application-based questions adding a slight challenge.
- Which chapters had the highest weightage in NEET 2025 Biology?
Chapters like Genetics and Inheritance Biotechnology and Plant Kingdom had the highest number of questions.
- How were questions distributed between Class 11 and Class 12 in Biology?
The distribution was fairly balanced, with Botany emphasizing more on Class 12 whereas Zoology contained equal portions of Class 11 and class 12.
- Were NEET 2025 Biology questions based on NCERT textbooks?
Yes, the majority of questions were NCERT-based, though some required deeper conceptual understanding and application.
- What kinds of questions were asked in the NEET 2025 Biology paper?
The question paper consisted of single-select MCQs, assertion-reason, match-the-column, multi-statement, and some diagram-based questions, probing knowledge as well as reasoning ability.
NEET 2025 Biology Question Paper PDF Download for Code 45
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